ecl chemiluminescent substrate (Elabscience Biotechnology)
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Elabscience Biotechnology
ecl chemiluminescent substrate
Ecl Chemiluminescent Substrate, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/excellent+chemiluminescent+substrate+kit/Excellent+Chemiluminescent+Substrate+(ECL)Detection+Kit/pmc13682527-52-37-45
Average 96 stars, based on 126 article reviews
Ecl Chemiluminescent Substrate, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/excellent+chemiluminescent+substrate+kit/Excellent+Chemiluminescent+Substrate+(ECL)Detection+Kit/pmc13682527-52-37-45
Average 96 stars, based on 126 article reviews
ecl chemiluminescent substrate - by Bioz Stars,
2026-11
96/100 stars
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Incubation:Article Title: Detection and Proinflammatory Effects of BTEX within the Human Atherosclerotic Plaque Article Snippet: Then, membranes were incubated overnight at 4 °C with specific primary antibodies: antitumor necrosis factor alpha (TNF-α, 1:1000, ab6671, Abcam, Cambridge, UK), anti-NLR Family Pyrin Domain Containing 3 (NLRP3, 1:1000, ab270449, Abcam, Cambridge, UK), and anti-IL-1β (1:1000, ab216995, Abcam, Cambridge, UK). .. After 1 h of incubation with peroxidase-conjugated secondary antibodies, immunocomplexes were revealed on dried membranes by using an Article Title: l-Carnitine and Acetyl-l-Carnitine Induce Metabolism Alteration and Mitophagy-Related Cell Death in Colorectal Cancer Cells. Article Snippet: After treatment, the HT-29 and HCT 116 cells were lysed in RIPA lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS in PBS), and the proteins were separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), and after blocking incubated at 4 ◦C overnight with specific primary antibodies: anti-SIRT4 (1:1000, ab231137, Abcam, Cambridge, UK), anti-5′ AMP-activated protein kinase alpha-1 (AMPK, 1:1000, Invitrogen, Waltham, MA, USA), anti-phospho-AMPK (1:1000, Invitrogen, Waltham, MA, USA), antiParkin (1:2000, ab77924, Abcam, Cambridge, UK), anti-PINK1 (1:500, Y403614, Applied Nutrients 2025, 17, 1010 5 of 16 Biological Materials Inc., Richmond, BC, Canada), anti-microtubule-associated protein 1 light chain 3B (LC3B, 1:1000, Abcam, Cambridge, UK), anti-α-tubulin (1:3000, E-AB-20036, Elabscience Biotechnology Inc., Houston, TX, USA), anti-actin (1:5000, Abcam, Cambridge, UK), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:2000, ab9485, Abcam, Cambridge, UK). .. After incubation with secondary antibodies, the immunocomplexes were examined with the Article Title: Detection and Proinflammatory Effects of BTEX within the Human Atherosclerotic Plaque. Article Snippet: XXXX, XXX, XXX−XXX C Cambridge, UK), and anti-IL-1β (1:1000, ab216995, Abcam, Cambridge, UK). .. After 1 h of incubation with peroxidaseconjugated secondary antibodies, immunocomplexes were revealed on dried membranes by using an Imaging:Article Title: Detection and Proinflammatory Effects of BTEX within the Human Atherosclerotic Plaque Article Snippet: Then, membranes were incubated overnight at 4 °C with specific primary antibodies: antitumor necrosis factor alpha (TNF-α, 1:1000, ab6671, Abcam, Cambridge, UK), anti-NLR Family Pyrin Domain Containing 3 (NLRP3, 1:1000, ab270449, Abcam, Cambridge, UK), and anti-IL-1β (1:1000, ab216995, Abcam, Cambridge, UK). .. After 1 h of incubation with peroxidase-conjugated secondary antibodies, immunocomplexes were revealed on dried membranes by using an Article Title: PCSK9 inhibition ameliorates microplastic-induced endothelial redox imbalance via SIRT6 modulation Article Snippet: Actin (1:2000, ab179467, Abcam, Cambridge, UK) and α-tubulin (1:2000, E-AB-20036, Elabscience Biotechnology Inc., Houston, TX, USA) were used as loading controls in different western blot experiments, depending on the experimental setting. .. Immunocomplexes were revealed using the Article Title: l-Carnitine and Acetyl-l-Carnitine Induce Metabolism Alteration and Mitophagy-Related Cell Death in Colorectal Cancer Cells. Article Snippet: After treatment, the HT-29 and HCT 116 cells were lysed in RIPA lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS in PBS), and the proteins were separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), and after blocking incubated at 4 ◦C overnight with specific primary antibodies: anti-SIRT4 (1:1000, ab231137, Abcam, Cambridge, UK), anti-5′ AMP-activated protein kinase alpha-1 (AMPK, 1:1000, Invitrogen, Waltham, MA, USA), anti-phospho-AMPK (1:1000, Invitrogen, Waltham, MA, USA), antiParkin (1:2000, ab77924, Abcam, Cambridge, UK), anti-PINK1 (1:500, Y403614, Applied Nutrients 2025, 17, 1010 5 of 16 Biological Materials Inc., Richmond, BC, Canada), anti-microtubule-associated protein 1 light chain 3B (LC3B, 1:1000, Abcam, Cambridge, UK), anti-α-tubulin (1:3000, E-AB-20036, Elabscience Biotechnology Inc., Houston, TX, USA), anti-actin (1:5000, Abcam, Cambridge, UK), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:2000, ab9485, Abcam, Cambridge, UK). .. After incubation with secondary antibodies, the immunocomplexes were examined with the Article Title: Detection and Proinflammatory Effects of BTEX within the Human Atherosclerotic Plaque. Article Snippet: XXXX, XXX, XXX−XXX C Cambridge, UK), and anti-IL-1β (1:1000, ab216995, Abcam, Cambridge, UK). .. After 1 h of incubation with peroxidaseconjugated secondary antibodies, immunocomplexes were revealed on dried membranes by using an Software:Article Title: Detection and Proinflammatory Effects of BTEX within the Human Atherosclerotic Plaque Article Snippet: Then, membranes were incubated overnight at 4 °C with specific primary antibodies: antitumor necrosis factor alpha (TNF-α, 1:1000, ab6671, Abcam, Cambridge, UK), anti-NLR Family Pyrin Domain Containing 3 (NLRP3, 1:1000, ab270449, Abcam, Cambridge, UK), and anti-IL-1β (1:1000, ab216995, Abcam, Cambridge, UK). .. After 1 h of incubation with peroxidase-conjugated secondary antibodies, immunocomplexes were revealed on dried membranes by using an Article Title: PCSK9 inhibition ameliorates microplastic-induced endothelial redox imbalance via SIRT6 modulation Article Snippet: Actin (1:2000, ab179467, Abcam, Cambridge, UK) and α-tubulin (1:2000, E-AB-20036, Elabscience Biotechnology Inc., Houston, TX, USA) were used as loading controls in different western blot experiments, depending on the experimental setting. .. Immunocomplexes were revealed using the Article Title: l-Carnitine and Acetyl-l-Carnitine Induce Metabolism Alteration and Mitophagy-Related Cell Death in Colorectal Cancer Cells. Article Snippet: After treatment, the HT-29 and HCT 116 cells were lysed in RIPA lysis buffer (1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS in PBS), and the proteins were separated via sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA), and after blocking incubated at 4 ◦C overnight with specific primary antibodies: anti-SIRT4 (1:1000, ab231137, Abcam, Cambridge, UK), anti-5′ AMP-activated protein kinase alpha-1 (AMPK, 1:1000, Invitrogen, Waltham, MA, USA), anti-phospho-AMPK (1:1000, Invitrogen, Waltham, MA, USA), antiParkin (1:2000, ab77924, Abcam, Cambridge, UK), anti-PINK1 (1:500, Y403614, Applied Nutrients 2025, 17, 1010 5 of 16 Biological Materials Inc., Richmond, BC, Canada), anti-microtubule-associated protein 1 light chain 3B (LC3B, 1:1000, Abcam, Cambridge, UK), anti-α-tubulin (1:3000, E-AB-20036, Elabscience Biotechnology Inc., Houston, TX, USA), anti-actin (1:5000, Abcam, Cambridge, UK), and anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH, 1:2000, ab9485, Abcam, Cambridge, UK). .. After incubation with secondary antibodies, the immunocomplexes were examined with the Article Title: Detection and Proinflammatory Effects of BTEX within the Human Atherosclerotic Plaque. Article Snippet: XXXX, XXX, XXX−XXX C Cambridge, UK), and anti-IL-1β (1:1000, ab216995, Abcam, Cambridge, UK). .. After 1 h of incubation with peroxidaseconjugated secondary antibodies, immunocomplexes were revealed on dried membranes by using an chemiluminescence:Article Title: Combining AdipoRon with Paclitaxel Unveils Synergistic Potential in Non-Small Cell Lung Cancer Cells via AMPK-ERK1/2 Signaling. Article Snippet: .. AdipoRon (#57583; Cell Signaling Technology, Danvers, MA, USA), Paclitaxel (#9807; Cell Signaling Technology, Danvers, MA, USA), Compound-C (EMD Millipore Corp., Burlington, MA, USA), PD98059 (P215; Sigma-Aldrich, St. Louis, MO, USA), Ethanol absolute an- hydrous (308603; Carlo Erba, Cornaredo, Italy), MTT solution (M2128; SigmaAldrich, St. Louis, MO, USA), Tween 20 (TC287; HIMEDIA, Mumbai, India), Phosphatebuffer solution (PBS) (ECB4004L; EuroClone, Pero, Italy), Propidium Iodide (#P4864; SigmaAldrich, St. Louis, MO, USA), RNase A (R5503; Sigma-Aldrich, St. Louis, MO, USA), RIPA buffer (R0278; Sigma-Aldrich, St. Louis, MO, USA), Protease/Phosphatase Inhibitor Cocktail (#5872; Cell Signaling Technology, Danvers, MA, USA), Trypsin-EDTA 1X in PBS (ECB3052D; EuroClone, Pero, Italy), 2X Laemmli (S3401; Sigma-Aldrich, St. Louis, MO, USA), Ponceau staining solution (A2935; PanReac AppliChem, Castellar del Vallès, Spain), Crystal violet (#C0775; Sigma-Aldrich, St. Louis, MO, USA), DMSO (A3672; PanReac AppliChem), Non-fat dry milk (A0530; PanReac AppliChem, Castellar del Vallès, Spain), Amersham Protran Premium 0.45 μm nitrocellulose membrane (10600008; Cytiva, Marlborough, MA, USA), and Excellent Chemiluminescent Substrate Kit (E-IR-R301; Elabsciences, Houston, TX, USA). .. |